13C E-protein spectrum

1H,13C TROSY-HMQC spectrum of E-protein full length

400 μM of ILVM-methyl 1H13C, U-2H15N labeled E-protein full length (1-75) in 20 mM deuterated HEPES pH 7.0, 100 mM NaCl, 1mM DTT, DDM and 10% D2O, measured at 298 K on a 600 MHz spectrometer equipped with TXI cryogenic probe.