13C N-protein NTD spectrum

1H,13C TROSY-HMQC spectrum of N-protein NTD (41-174)

55 μM of ILVM-methyl 1H13C, U-2H15N labeled N-protein NTD (41-174) in 20 mM deuterated HEPES pH 7.0, 100 mM NaCl, 1mM DTT and 10% D2O, measured at 298 K on a 600 MHz spectrometer equipped with TXI cryogenic probe.