13C Nsp4 spectrum

1H,13C TROSY-HMQC spectrum of Nsp4 (401-500)

71 μM of ILVM-methyl 1H13C, U-2H15N labeled Nsp4 (401-500) in 20 mM deuterated HEPES pH 7.0, 100 mM NaCl, 1mM DTT, and 10% D2O, measured at 298 K on a 600 MHz spectrometer equipped with TXI cryogenic probe.