13C S-protein E484K mutation spectrum

1H,13C TROSY-HMQC spectrum of S-protein RBD (330-532) with E484K mutation

25 μM of ILVM-methyl 1H13C, U-2H15N labeled S-protein RBD (330-532) with E484K mutation in 20 mM deuterated HEPES pH 7.0, 100 mM NaCl and 10% D2O, measured at 298 K on a 600 MHz spectrometer equipped with TXI cryogenic probe.