15N Nsp1 spectrum

1H,15N TROSY-HSQC spectrum of N-protein CTD (247-364)

63 μM of ILVM-methyl 1H13C, U-2H15N labeled N-protein CTD (247-364) in 20 mM deuterated HEPES pH 7.0, 100 mM NaCl, 1mM DTT and 10% D2O, measured at 298 K on a 600 MHz spectrometer equipped with TXI cryogenic probe.