15N Nsp3_1-112 spectrum

1H,15N TROSY-HSQC spectrum of Nsp3 (1-112)

54 μM of ILVM-methyl 1H13C, U-2H15N labeled Nsp3 (1-112) in 20 mM deuterated HEPES pH 7.0, 100 mM NaCl, 1mM DTT, and 10% D2O, measured at 298 K on a 600 MHz spectrometer equipped with TXI cryogenic probe.